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reverse transcription kit  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co reverse transcription kit
    Reverse Transcription Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 10630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcription+kit/HiScript+III+RT+SuperMix+for+qPCR+%2BgDNA+wiper/10__1093_slash_rb_slash_rbag218-114-26-30
    Average 99 stars, based on 10630 article reviews
    reverse transcription kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Circular RNA-mediated regulatory networks drive oxidative stress and metabolic dysregulation in broiler breast muscle under chronic heat stress.
    Article Snippet: Chronic heat stress (CHS) disrupts broiler breast muscle physiology homeostasis, yet the underlying molecular mechanisms remain unclear.. In this study, 144 male Arbor Acres broilers (28 d old) were randomly allocated into 3 treatment groups: the normal control (NC) group (22 C, ad libitum), the heat stress (HS) group (22 C, ad libitum), the pair-fed (PF) group (22 C, feed matched to HS).. After two weeks of CHS, the HS group exhibited elevated ROS production, increased antioxidant enzyme activities (GSH-Px, CAT, SOD), and higher MDA and carbonyl levels, accompanied by pronounced ultrastructural damage in mitochondria and myofibrils.

    Article Title: Emerging roles for SNRPB2 in governing the cell cycle and steering tumor immune modulation in breast cancer
    Article Snippet: Total RNA was isolated from MDA-MB-231 cells using TRIzol reagent (15596-018, Ambion, USA). .. RNA quantity and purity were measured using an Ultrafine spectrophotometer (N50 touch, IMPLEN, Germany), and RNA integrity was further verified by 1.0% agarose gel electrophoresis. cDNA was synthesized using a reverse transcription kit (R323-01, Vazyme, China) on a thermocycler (T100, Bio-Rad, USA), followed by qPCR on the ABI QuantStudio5. ..

    Article Title: T-2 toxin induces METTL21A-mediated BiP trimethylation under endoplasmic reticulum stress
    Article Snippet: T-2 toxin, a prevalent cereal contaminant, threatens human and animal health.. The immunoglobulin heavy chain binding protein (BiP), an endoplasmic reticulum (ER) chaperone, is a key regulator of the unfolded protein response (UPR) under ER stress.. Accurate detection of BiP is critical for food safety and toxicological studies, as ER stress is involved in multiple diseases and toxicological effects.

    Article Title: TRPV4-linked oxidative–lysosomal stress in myocardial ischemia/reperfusion: disease-matched transcriptomics and functional analysis in OGD/R cardiomyoblasts
    Article Snippet: Total RNA was extracted from H9C2 Cardiomyoblasts using the FreeZol Reagent Kit (R711-01, Vazyme Biotech, China). .. Complementary DNA (cDNA) was synthesized using a reverse transcription kit (R323-01, Vazyme Biotech, China). .. qRT-PCR was performed with ChamQ Universal SYBR qPCR Master Mix (Q711-02, Vazyme Biotech, China).

    Reverse Transcription:

    Article Title: Circular RNA-mediated regulatory networks drive oxidative stress and metabolic dysregulation in broiler breast muscle under chronic heat stress.
    Article Snippet: Chronic heat stress (CHS) disrupts broiler breast muscle physiology homeostasis, yet the underlying molecular mechanisms remain unclear.. In this study, 144 male Arbor Acres broilers (28 d old) were randomly allocated into 3 treatment groups: the normal control (NC) group (22 C, ad libitum), the heat stress (HS) group (22 C, ad libitum), the pair-fed (PF) group (22 C, feed matched to HS).. After two weeks of CHS, the HS group exhibited elevated ROS production, increased antioxidant enzyme activities (GSH-Px, CAT, SOD), and higher MDA and carbonyl levels, accompanied by pronounced ultrastructural damage in mitochondria and myofibrils.

    Article Title: Emerging roles for SNRPB2 in governing the cell cycle and steering tumor immune modulation in breast cancer
    Article Snippet: Total RNA was isolated from MDA-MB-231 cells using TRIzol reagent (15596-018, Ambion, USA). .. RNA quantity and purity were measured using an Ultrafine spectrophotometer (N50 touch, IMPLEN, Germany), and RNA integrity was further verified by 1.0% agarose gel electrophoresis. cDNA was synthesized using a reverse transcription kit (R323-01, Vazyme, China) on a thermocycler (T100, Bio-Rad, USA), followed by qPCR on the ABI QuantStudio5. ..

    Article Title: Down-regulation of RhoA inhibits YAP by activating Hippo signaling to promote macrophage M1 polarization and enhance its phagocytosis, thereby inhibiting AML progression in preclinical models.
    Article Snippet: 159 2.3 RT-qPCR 160 AML cells (MOLM-13, Kasumi-1, KG-1, HL-60) and CD34+ cells were 161 collected and total RNA was extracted through Trizol reagent (15596026CN, Thermo 162 Fisher Scientific). .. Reverse transcription kit (R333-01, Vazyme, Nanjing, China) 163 J urn al Pr e-p roo f 7 reversed transcribe 1 μg of total RNA into cDNA following kit instructions. .. 164 Fluorescence quantitative PCR kit (RR420A, Takara, Tokyo, Japan) was used for 165 amplification Reaction conditions: 95°C for 30 s pre-denaturation; 95°C for 5 s, 60°C 166 for 30 s annealing and extension, a total of 40 cycles.

    Article Title: T-2 toxin induces METTL21A-mediated BiP trimethylation under endoplasmic reticulum stress
    Article Snippet: T-2 toxin, a prevalent cereal contaminant, threatens human and animal health.. The immunoglobulin heavy chain binding protein (BiP), an endoplasmic reticulum (ER) chaperone, is a key regulator of the unfolded protein response (UPR) under ER stress.. Accurate detection of BiP is critical for food safety and toxicological studies, as ER stress is involved in multiple diseases and toxicological effects.

    Article Title: TRPV4-linked oxidative–lysosomal stress in myocardial ischemia/reperfusion: disease-matched transcriptomics and functional analysis in OGD/R cardiomyoblasts
    Article Snippet: Total RNA was extracted from H9C2 Cardiomyoblasts using the FreeZol Reagent Kit (R711-01, Vazyme Biotech, China). .. Complementary DNA (cDNA) was synthesized using a reverse transcription kit (R323-01, Vazyme Biotech, China). .. qRT-PCR was performed with ChamQ Universal SYBR qPCR Master Mix (Q711-02, Vazyme Biotech, China).

    Article Title: Lotus leaf-inspired radially aligned PHBV dressing with herbal delivery and ultrasound-responsive piezoelectricity for infected diabetic wound healing
    Article Snippet: Fluorescence images were acquired using a confocal laser scanning microscope (CLSM, LSM 900, Carl Zeiss, Germany). .. ECM gene expression analysis: Total RNA was extracted using an RNA extraction kit (RC101, Nanjing Vazyme Biotech Co., Ltd., China) and reverse-transcribed into cDNA using a reverse-transcription kit (R323, Nanjing Vazyme Biotech Co., Ltd., China). .. Gene expression analysis was performed using specific primers and Universal SYBR Green PCR Master Mix (RR820A, Takara, Japan) on a LightCycler 480 Real-Time PCR System (Roche, Switzerland).

    other:

    Article Title: Immediate protective effect of rUCMSC-EVs on ovarian function in a cyclophosphamide -induced premature ovarian insufficiency rats: counteracting granulosa cell apoptosis.
    Article Snippet: Real‐Time PCR Total RNA was extracted from ovarian tissue using TRIzol reagent (98597501, Invitrogen), and its concentration was quantified with a Nanodrop spectrophotometer (Thermo Fisher Scientific).

    Article Title: NLRP6 governs hippocampal microglial phagocytosis to resist stress-induced depressive-like behaviors in mice and the intervention effect of bezafibrate
    Article Snippet: The supernatant was collected, and the total intracellular ATP content was determined using an ATP assay kit (S0026, Beyotime, China) following manufacturer instructions.

    Spectrophotometry:

    Article Title: Emerging roles for SNRPB2 in governing the cell cycle and steering tumor immune modulation in breast cancer
    Article Snippet: Total RNA was isolated from MDA-MB-231 cells using TRIzol reagent (15596-018, Ambion, USA). .. RNA quantity and purity were measured using an Ultrafine spectrophotometer (N50 touch, IMPLEN, Germany), and RNA integrity was further verified by 1.0% agarose gel electrophoresis. cDNA was synthesized using a reverse transcription kit (R323-01, Vazyme, China) on a thermocycler (T100, Bio-Rad, USA), followed by qPCR on the ABI QuantStudio5. ..

    Agarose Gel Electrophoresis:

    Article Title: Emerging roles for SNRPB2 in governing the cell cycle and steering tumor immune modulation in breast cancer
    Article Snippet: Total RNA was isolated from MDA-MB-231 cells using TRIzol reagent (15596-018, Ambion, USA). .. RNA quantity and purity were measured using an Ultrafine spectrophotometer (N50 touch, IMPLEN, Germany), and RNA integrity was further verified by 1.0% agarose gel electrophoresis. cDNA was synthesized using a reverse transcription kit (R323-01, Vazyme, China) on a thermocycler (T100, Bio-Rad, USA), followed by qPCR on the ABI QuantStudio5. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Emerging roles for SNRPB2 in governing the cell cycle and steering tumor immune modulation in breast cancer
    Article Snippet: Total RNA was isolated from MDA-MB-231 cells using TRIzol reagent (15596-018, Ambion, USA). .. RNA quantity and purity were measured using an Ultrafine spectrophotometer (N50 touch, IMPLEN, Germany), and RNA integrity was further verified by 1.0% agarose gel electrophoresis. cDNA was synthesized using a reverse transcription kit (R323-01, Vazyme, China) on a thermocycler (T100, Bio-Rad, USA), followed by qPCR on the ABI QuantStudio5. ..

    Gene Expression:

    Article Title: Lotus leaf-inspired radially aligned PHBV dressing with herbal delivery and ultrasound-responsive piezoelectricity for infected diabetic wound healing
    Article Snippet: Fluorescence images were acquired using a confocal laser scanning microscope (CLSM, LSM 900, Carl Zeiss, Germany). .. ECM gene expression analysis: Total RNA was extracted using an RNA extraction kit (RC101, Nanjing Vazyme Biotech Co., Ltd., China) and reverse-transcribed into cDNA using a reverse-transcription kit (R323, Nanjing Vazyme Biotech Co., Ltd., China). .. Gene expression analysis was performed using specific primers and Universal SYBR Green PCR Master Mix (RR820A, Takara, Japan) on a LightCycler 480 Real-Time PCR System (Roche, Switzerland).

    Analysis:

    Article Title: Lotus leaf-inspired radially aligned PHBV dressing with herbal delivery and ultrasound-responsive piezoelectricity for infected diabetic wound healing
    Article Snippet: Fluorescence images were acquired using a confocal laser scanning microscope (CLSM, LSM 900, Carl Zeiss, Germany). .. ECM gene expression analysis: Total RNA was extracted using an RNA extraction kit (RC101, Nanjing Vazyme Biotech Co., Ltd., China) and reverse-transcribed into cDNA using a reverse-transcription kit (R323, Nanjing Vazyme Biotech Co., Ltd., China). .. Gene expression analysis was performed using specific primers and Universal SYBR Green PCR Master Mix (RR820A, Takara, Japan) on a LightCycler 480 Real-Time PCR System (Roche, Switzerland).

    RNA Extraction:

    Article Title: Lotus leaf-inspired radially aligned PHBV dressing with herbal delivery and ultrasound-responsive piezoelectricity for infected diabetic wound healing
    Article Snippet: Fluorescence images were acquired using a confocal laser scanning microscope (CLSM, LSM 900, Carl Zeiss, Germany). .. ECM gene expression analysis: Total RNA was extracted using an RNA extraction kit (RC101, Nanjing Vazyme Biotech Co., Ltd., China) and reverse-transcribed into cDNA using a reverse-transcription kit (R323, Nanjing Vazyme Biotech Co., Ltd., China). .. Gene expression analysis was performed using specific primers and Universal SYBR Green PCR Master Mix (RR820A, Takara, Japan) on a LightCycler 480 Real-Time PCR System (Roche, Switzerland).



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